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    Bio-Rad n8129 bio spin p 6 gel columns bio rad
    N8129 Bio Spin P 6 Gel Columns Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n8129+bio+spin+p+6+gel+columns+bio+rad/Bio-Spin+P-6+Gel+Columns/pm39842434-239-15-20
    Average 94 stars, based on 211 article reviews
    n8129 bio spin p 6 gel columns bio rad - by Bioz Stars, 2026-09
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    Recombinant:

    Article Title: Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triethylammonium bicarbonate buffer Sigma Aldrich Cat# 18597 Hydroxylamine solution Sigma Aldrich Cat# 467804 Trypsin Promega Cat# V5117 TMT10plex Thermo Scientific Cat# 90110 DCP-Bio1 Merck Cat# NS1226 Copper (II)-TBTA complex Lumiprobe Cat# 21050 L-ascorbic acid Sigma-Aldrich Cat# A92902 Formic acid Suprapur Supelco Cat# 111670 TCEP Sigma-Aldrich Cat# 646547 AQC Synchem Cat# s041 AccQ-Tag Ultra Borate Buffer Waters Cat# 186009283 Cyanine5 alkyne Lumirpobe Cat# A30B0 Laemmli (4X) buffer BioRad Cat# 1610747 b-mercaptoethanol Sigma Aldrich Cat# M3148 Western Blotting Luminol Reagent Santa Cruz Biotechnology Cat# sc-2048 Ponceau S Sigma Aldrich Cat# P3504 Hydrogen Peroxide Solution Sigma Aldrich Cat# 216763 Erastin Sigma Aldrich Cat# E7781 1S,3R-RSL 3 Sigma Aldrich Cat# SML2234 Thiazolyl Blue Tetrazolium Bromide Sigma Aldrich Cat# M5655 Methyl viologen dichloride hydrate Sigma Aldrich Cat# 856177 Sodium (meta)arsenite Santa Cruz Biotechnology Cat# sc-250986 Nile Red Sigma Aldrich Cat# 72485 Oil Red O Sigma Aldrich Cat# O0625 Bovine Serum Albumin Sigma Aldrich Cat# A7906 L-(+)-Ergothioneine Thermo Scientific Cat# J67861.03 L-Hercynine Sigma Aldrich Cat# SMB00978 DMEM Gibco Cat# 21969-035 DMEM, high glucose, no glutamine, no methionine, no cystine Gibco Cat# 21969-035 DMEM/F-12 Gibco Cat# 21041025 DMEM, no phenol-red Gibco Cat# 31053028 Endothelial Cell Growth Medium 2 (ECGM2) PromoCell Cat# C-22011 Fetal bovine serum Pan Biotech Cat# P30-3031 L-glutamine Pan Biotech Cat# P64-80100 Penicillin-streptomycin Pan Biotech Cat# P66-07100 Polybrene Santa Cruz Biotechnology Cat# 28728-55-4 Lipofectamine RNAiMAX Invitrogen Cat# 13778075 DPBS Sigma Aldrich Cat# D8537 Hoechst 33342 Sigma Aldrich Cat# B2261 L-Cystine Sigma Aldrich Cat# 30200 Pierce High Capacity NeutrAvidin Agarose beads Thermofisher Cat# 20357 Pierce NeutrAvidin Agarose beads Thermofisher Cat# 29200 DTAB Thermo Scientific Cat# A10761.09 Sodium disulfide Dojindo Molecular Technologies Cat# SB-02 Dihydroxyacetone phosphate hemimagnesium salt hydrate Sigma Aldrich Cat# 51269 (Continued on next page) Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER b-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate Sigma Aldrich Cat# N8129 Bio-Spin P-6 Gel Columns Bio-Rad Cat# 7326227 Lead(II) acetate trihydrate Thermo Scientific Acros Cat# 423841000 Pyridoxal 5’-phosphate hydrate Thermo Scientific Cat# A12323 Granulated agar BD Difco Cat# BD 214530 Bacto Peptone Gibco Cat# 211677 LB Broth (Lennox) Carl Roth Cat# X964.1 NaCl Carl-Roth Cat# HN00.2 Potassium dihydrogen phosphate Carl-Roth Cat# P018.2 Di-sodium hydrogen phosphate dihydrate Carl-Roth Cat# 4984.1 Potassium phosphate dibasic Euromedex Cat# PB0447-B Agarose D5 Euromedex Cat# D5-C Ethanol Fisher BioReagents Cat# BP2818500 TRIzol Reagent Invitrogen Cat# 15596026 Phenol:chloroform:isoamyl alcohol mix (25:24:1) Sigma Aldrich Cat# 77617 DMSO Carl-Roth Cat# 4720.3 Methanol, Optima LC/MS Grade Fisher Scientific Cat# A456-212 Trichloromethane/Chloroform Carl-Roth Cat# 7331.1 Acetone ROTIPURAN R99.8% Carl-Roth Cat# 9372.2 Acetonitrile Biosolve Cat# 00012041001BS Acetic acid ThermoFisher Cat# A/0406/PB15 Isopropanol Fisher Chemicals P/7507/17 Ammonia solution 28-30% Sigma Aldrich Cat# 5.33003 NaOH Carl-Roth Cat# 1CCX.2 Hydrochloric acid 1N Carl-Roth Cat# K025.1 Trifluoroacetic acid Biosolve Cat# 00202341A8BS Glycerol-3-phosphate dehydrogenase (GDH) Roche Cat# 10127752001 GST protein, tag-free Sigma Aldrich Cat# SRP5348 Human recombinant CSE, GST-tagged From Prof. Bibli Hu et al.66 Human recombinant CSE From Prof. Ruma Banarjee Wedmann et al.67 BODIPY 581/591 C11 Invitrogen Cat# D3861 SF7-AM Cayman Chemicals Cat# 14623 Critical commercial assays DC protein assay Biorad Cat# 5000112 NAD/NADH-Glo Promega Cat# G9081 NADP/NADPH-Glo Promega Cat# G9071 PureLink RNA Mini Kit Invitrogen Cat# 12183018A Glycerol-3-Phosphate Assay Kit Sigma Aldrich Cat# MAK207 L-Lactate Assay Kit Sigma Aldrich Cat# MAK443 Oxaloacetate Assay Kit Sigma Aldrich Cat# MAK070 Pyruvate Assay Kit Sigma Aldrich Cat# MAK071 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific Cat# 84868 Deposited data Total proteome, persulfidome; Thermal proteome profiling data PRIDE repository PXD051587; PXD051609 (Continued on next page) e3 Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNA Seq data BioProject database PRJNA1178314 Raw data for graphs This paper Data S1 Experimental models: Cell lines Mouse: MEF WT (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Mouse: MEF CSE-/- (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Human: HUVEC N/A Human: HEK293T N/A Experimental models: Organisms/strains C. elegans Wild-Type Bristol N2 Caenorhabditis Genetic Center N/A C. elegans: strain MRF03 (cth-1(ok3319)V) Caenorhabditis Genetic Center strain VC2569 C. elegans: strain MRF09 mpst-3(tm4387)V MITANI Lab, National Bio-Resource; Project of the MEXT, Japan strain FX04387 C. elegans: strain CF1038 (daf-16 (mu86)I) Caenorhabditis Genetic Center N/A C. elegans: strain SJ4103 (zcIs14 [Pmyo-3::mitoGFP]) From A.M. Vizuete (The Institute of Biomedicine of Seville) N/A C. elegans: strain RB1373 (F47G4.3(ok1558) I) Caenorhabditis Genetic Center N/A C. elegans: strain RB1482 (K11H3.1(ok1733) III) Caenorhabditis Genetic Center N/A Male Wistar Rat Institute for Biological Research ‘‘Sinisa Stankovic’’, Belgrade, Serbia N/A Oligonucleotides siRNA directed against human CSE Eurogentec custom synthesis Recombinant DNA psPAX2 Didier Trono Lab Addgene Plasimd #12260 pMD2.G Didier Trono Lab Addgene Plasimd #12259 Cdt-mKO Riken, Japan Geminin-GFP Riken, Japan Software and algorithms R Environment The R Project for Statistical Computing https://www.r-project.org/ R studio RStudio, Inc https://www.rstudio.com/ PEAKS ONLINE Bioinformatics Solutions Inc. http://www.bioinfor.com/ ImageJ Schneider et al.69 https://imagej.nih.gov/ij/download.html Fiji Schneider et al.69 https://imagej.net/software/fiji/ BioVenn Hulsen et al.70 https://www.biovenn.nl/ GraphPad Prism 10.2.0 GraphPad Software https://www.graphpad.com FragPipe Kong et al.71 https://fragpipe.nesvilab.org/ DAVID Bioinformatics Database v6.8 Huang et al.72 https://david.ncifcrf.gov/ EigenMS R script Karpievitch et al.73 https://rdrr.io/bioc/ProteoMM/src/ R/EigenMS.R Origin, Version 2022 OriginLab Corporation, Northampton, MA, USA N/A ChemDraw 20.0.0.38 PerkinElmer Informatics, Inc N/A Adobe Illustrator 28.4.1 Adobe https://www.adobe.com/products/ illustrator.html BioRender https://www.biorender.com/ Oasis Yang et al.74 https://sbi.postech.ac.kr/oasis/ CellProfiler Stirling et al.75 SeamDock Murail et al.29 https://bioserv.rpbs.univ-paris-diderot.fr/ services/SeamDock/ Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e4

    DC Protein Assay:

    Article Title: Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triethylammonium bicarbonate buffer Sigma Aldrich Cat# 18597 Hydroxylamine solution Sigma Aldrich Cat# 467804 Trypsin Promega Cat# V5117 TMT10plex Thermo Scientific Cat# 90110 DCP-Bio1 Merck Cat# NS1226 Copper (II)-TBTA complex Lumiprobe Cat# 21050 L-ascorbic acid Sigma-Aldrich Cat# A92902 Formic acid Suprapur Supelco Cat# 111670 TCEP Sigma-Aldrich Cat# 646547 AQC Synchem Cat# s041 AccQ-Tag Ultra Borate Buffer Waters Cat# 186009283 Cyanine5 alkyne Lumirpobe Cat# A30B0 Laemmli (4X) buffer BioRad Cat# 1610747 b-mercaptoethanol Sigma Aldrich Cat# M3148 Western Blotting Luminol Reagent Santa Cruz Biotechnology Cat# sc-2048 Ponceau S Sigma Aldrich Cat# P3504 Hydrogen Peroxide Solution Sigma Aldrich Cat# 216763 Erastin Sigma Aldrich Cat# E7781 1S,3R-RSL 3 Sigma Aldrich Cat# SML2234 Thiazolyl Blue Tetrazolium Bromide Sigma Aldrich Cat# M5655 Methyl viologen dichloride hydrate Sigma Aldrich Cat# 856177 Sodium (meta)arsenite Santa Cruz Biotechnology Cat# sc-250986 Nile Red Sigma Aldrich Cat# 72485 Oil Red O Sigma Aldrich Cat# O0625 Bovine Serum Albumin Sigma Aldrich Cat# A7906 L-(+)-Ergothioneine Thermo Scientific Cat# J67861.03 L-Hercynine Sigma Aldrich Cat# SMB00978 DMEM Gibco Cat# 21969-035 DMEM, high glucose, no glutamine, no methionine, no cystine Gibco Cat# 21969-035 DMEM/F-12 Gibco Cat# 21041025 DMEM, no phenol-red Gibco Cat# 31053028 Endothelial Cell Growth Medium 2 (ECGM2) PromoCell Cat# C-22011 Fetal bovine serum Pan Biotech Cat# P30-3031 L-glutamine Pan Biotech Cat# P64-80100 Penicillin-streptomycin Pan Biotech Cat# P66-07100 Polybrene Santa Cruz Biotechnology Cat# 28728-55-4 Lipofectamine RNAiMAX Invitrogen Cat# 13778075 DPBS Sigma Aldrich Cat# D8537 Hoechst 33342 Sigma Aldrich Cat# B2261 L-Cystine Sigma Aldrich Cat# 30200 Pierce High Capacity NeutrAvidin Agarose beads Thermofisher Cat# 20357 Pierce NeutrAvidin Agarose beads Thermofisher Cat# 29200 DTAB Thermo Scientific Cat# A10761.09 Sodium disulfide Dojindo Molecular Technologies Cat# SB-02 Dihydroxyacetone phosphate hemimagnesium salt hydrate Sigma Aldrich Cat# 51269 (Continued on next page) Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER b-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate Sigma Aldrich Cat# N8129 Bio-Spin P-6 Gel Columns Bio-Rad Cat# 7326227 Lead(II) acetate trihydrate Thermo Scientific Acros Cat# 423841000 Pyridoxal 5’-phosphate hydrate Thermo Scientific Cat# A12323 Granulated agar BD Difco Cat# BD 214530 Bacto Peptone Gibco Cat# 211677 LB Broth (Lennox) Carl Roth Cat# X964.1 NaCl Carl-Roth Cat# HN00.2 Potassium dihydrogen phosphate Carl-Roth Cat# P018.2 Di-sodium hydrogen phosphate dihydrate Carl-Roth Cat# 4984.1 Potassium phosphate dibasic Euromedex Cat# PB0447-B Agarose D5 Euromedex Cat# D5-C Ethanol Fisher BioReagents Cat# BP2818500 TRIzol Reagent Invitrogen Cat# 15596026 Phenol:chloroform:isoamyl alcohol mix (25:24:1) Sigma Aldrich Cat# 77617 DMSO Carl-Roth Cat# 4720.3 Methanol, Optima LC/MS Grade Fisher Scientific Cat# A456-212 Trichloromethane/Chloroform Carl-Roth Cat# 7331.1 Acetone ROTIPURAN R99.8% Carl-Roth Cat# 9372.2 Acetonitrile Biosolve Cat# 00012041001BS Acetic acid ThermoFisher Cat# A/0406/PB15 Isopropanol Fisher Chemicals P/7507/17 Ammonia solution 28-30% Sigma Aldrich Cat# 5.33003 NaOH Carl-Roth Cat# 1CCX.2 Hydrochloric acid 1N Carl-Roth Cat# K025.1 Trifluoroacetic acid Biosolve Cat# 00202341A8BS Glycerol-3-phosphate dehydrogenase (GDH) Roche Cat# 10127752001 GST protein, tag-free Sigma Aldrich Cat# SRP5348 Human recombinant CSE, GST-tagged From Prof. Bibli Hu et al.66 Human recombinant CSE From Prof. Ruma Banarjee Wedmann et al.67 BODIPY 581/591 C11 Invitrogen Cat# D3861 SF7-AM Cayman Chemicals Cat# 14623 Critical commercial assays DC protein assay Biorad Cat# 5000112 NAD/NADH-Glo Promega Cat# G9081 NADP/NADPH-Glo Promega Cat# G9071 PureLink RNA Mini Kit Invitrogen Cat# 12183018A Glycerol-3-Phosphate Assay Kit Sigma Aldrich Cat# MAK207 L-Lactate Assay Kit Sigma Aldrich Cat# MAK443 Oxaloacetate Assay Kit Sigma Aldrich Cat# MAK070 Pyruvate Assay Kit Sigma Aldrich Cat# MAK071 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific Cat# 84868 Deposited data Total proteome, persulfidome; Thermal proteome profiling data PRIDE repository PXD051587; PXD051609 (Continued on next page) e3 Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNA Seq data BioProject database PRJNA1178314 Raw data for graphs This paper Data S1 Experimental models: Cell lines Mouse: MEF WT (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Mouse: MEF CSE-/- (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Human: HUVEC N/A Human: HEK293T N/A Experimental models: Organisms/strains C. elegans Wild-Type Bristol N2 Caenorhabditis Genetic Center N/A C. elegans: strain MRF03 (cth-1(ok3319)V) Caenorhabditis Genetic Center strain VC2569 C. elegans: strain MRF09 mpst-3(tm4387)V MITANI Lab, National Bio-Resource; Project of the MEXT, Japan strain FX04387 C. elegans: strain CF1038 (daf-16 (mu86)I) Caenorhabditis Genetic Center N/A C. elegans: strain SJ4103 (zcIs14 [Pmyo-3::mitoGFP]) From A.M. Vizuete (The Institute of Biomedicine of Seville) N/A C. elegans: strain RB1373 (F47G4.3(ok1558) I) Caenorhabditis Genetic Center N/A C. elegans: strain RB1482 (K11H3.1(ok1733) III) Caenorhabditis Genetic Center N/A Male Wistar Rat Institute for Biological Research ‘‘Sinisa Stankovic’’, Belgrade, Serbia N/A Oligonucleotides siRNA directed against human CSE Eurogentec custom synthesis Recombinant DNA psPAX2 Didier Trono Lab Addgene Plasimd #12260 pMD2.G Didier Trono Lab Addgene Plasimd #12259 Cdt-mKO Riken, Japan Geminin-GFP Riken, Japan Software and algorithms R Environment The R Project for Statistical Computing https://www.r-project.org/ R studio RStudio, Inc https://www.rstudio.com/ PEAKS ONLINE Bioinformatics Solutions Inc. http://www.bioinfor.com/ ImageJ Schneider et al.69 https://imagej.nih.gov/ij/download.html Fiji Schneider et al.69 https://imagej.net/software/fiji/ BioVenn Hulsen et al.70 https://www.biovenn.nl/ GraphPad Prism 10.2.0 GraphPad Software https://www.graphpad.com FragPipe Kong et al.71 https://fragpipe.nesvilab.org/ DAVID Bioinformatics Database v6.8 Huang et al.72 https://david.ncifcrf.gov/ EigenMS R script Karpievitch et al.73 https://rdrr.io/bioc/ProteoMM/src/ R/EigenMS.R Origin, Version 2022 OriginLab Corporation, Northampton, MA, USA N/A ChemDraw 20.0.0.38 PerkinElmer Informatics, Inc N/A Adobe Illustrator 28.4.1 Adobe https://www.adobe.com/products/ illustrator.html BioRender https://www.biorender.com/ Oasis Yang et al.74 https://sbi.postech.ac.kr/oasis/ CellProfiler Stirling et al.75 SeamDock Murail et al.29 https://bioserv.rpbs.univ-paris-diderot.fr/ services/SeamDock/ Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e4

    Pyruvate Assay:

    Article Title: Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triethylammonium bicarbonate buffer Sigma Aldrich Cat# 18597 Hydroxylamine solution Sigma Aldrich Cat# 467804 Trypsin Promega Cat# V5117 TMT10plex Thermo Scientific Cat# 90110 DCP-Bio1 Merck Cat# NS1226 Copper (II)-TBTA complex Lumiprobe Cat# 21050 L-ascorbic acid Sigma-Aldrich Cat# A92902 Formic acid Suprapur Supelco Cat# 111670 TCEP Sigma-Aldrich Cat# 646547 AQC Synchem Cat# s041 AccQ-Tag Ultra Borate Buffer Waters Cat# 186009283 Cyanine5 alkyne Lumirpobe Cat# A30B0 Laemmli (4X) buffer BioRad Cat# 1610747 b-mercaptoethanol Sigma Aldrich Cat# M3148 Western Blotting Luminol Reagent Santa Cruz Biotechnology Cat# sc-2048 Ponceau S Sigma Aldrich Cat# P3504 Hydrogen Peroxide Solution Sigma Aldrich Cat# 216763 Erastin Sigma Aldrich Cat# E7781 1S,3R-RSL 3 Sigma Aldrich Cat# SML2234 Thiazolyl Blue Tetrazolium Bromide Sigma Aldrich Cat# M5655 Methyl viologen dichloride hydrate Sigma Aldrich Cat# 856177 Sodium (meta)arsenite Santa Cruz Biotechnology Cat# sc-250986 Nile Red Sigma Aldrich Cat# 72485 Oil Red O Sigma Aldrich Cat# O0625 Bovine Serum Albumin Sigma Aldrich Cat# A7906 L-(+)-Ergothioneine Thermo Scientific Cat# J67861.03 L-Hercynine Sigma Aldrich Cat# SMB00978 DMEM Gibco Cat# 21969-035 DMEM, high glucose, no glutamine, no methionine, no cystine Gibco Cat# 21969-035 DMEM/F-12 Gibco Cat# 21041025 DMEM, no phenol-red Gibco Cat# 31053028 Endothelial Cell Growth Medium 2 (ECGM2) PromoCell Cat# C-22011 Fetal bovine serum Pan Biotech Cat# P30-3031 L-glutamine Pan Biotech Cat# P64-80100 Penicillin-streptomycin Pan Biotech Cat# P66-07100 Polybrene Santa Cruz Biotechnology Cat# 28728-55-4 Lipofectamine RNAiMAX Invitrogen Cat# 13778075 DPBS Sigma Aldrich Cat# D8537 Hoechst 33342 Sigma Aldrich Cat# B2261 L-Cystine Sigma Aldrich Cat# 30200 Pierce High Capacity NeutrAvidin Agarose beads Thermofisher Cat# 20357 Pierce NeutrAvidin Agarose beads Thermofisher Cat# 29200 DTAB Thermo Scientific Cat# A10761.09 Sodium disulfide Dojindo Molecular Technologies Cat# SB-02 Dihydroxyacetone phosphate hemimagnesium salt hydrate Sigma Aldrich Cat# 51269 (Continued on next page) Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER b-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate Sigma Aldrich Cat# N8129 Bio-Spin P-6 Gel Columns Bio-Rad Cat# 7326227 Lead(II) acetate trihydrate Thermo Scientific Acros Cat# 423841000 Pyridoxal 5’-phosphate hydrate Thermo Scientific Cat# A12323 Granulated agar BD Difco Cat# BD 214530 Bacto Peptone Gibco Cat# 211677 LB Broth (Lennox) Carl Roth Cat# X964.1 NaCl Carl-Roth Cat# HN00.2 Potassium dihydrogen phosphate Carl-Roth Cat# P018.2 Di-sodium hydrogen phosphate dihydrate Carl-Roth Cat# 4984.1 Potassium phosphate dibasic Euromedex Cat# PB0447-B Agarose D5 Euromedex Cat# D5-C Ethanol Fisher BioReagents Cat# BP2818500 TRIzol Reagent Invitrogen Cat# 15596026 Phenol:chloroform:isoamyl alcohol mix (25:24:1) Sigma Aldrich Cat# 77617 DMSO Carl-Roth Cat# 4720.3 Methanol, Optima LC/MS Grade Fisher Scientific Cat# A456-212 Trichloromethane/Chloroform Carl-Roth Cat# 7331.1 Acetone ROTIPURAN R99.8% Carl-Roth Cat# 9372.2 Acetonitrile Biosolve Cat# 00012041001BS Acetic acid ThermoFisher Cat# A/0406/PB15 Isopropanol Fisher Chemicals P/7507/17 Ammonia solution 28-30% Sigma Aldrich Cat# 5.33003 NaOH Carl-Roth Cat# 1CCX.2 Hydrochloric acid 1N Carl-Roth Cat# K025.1 Trifluoroacetic acid Biosolve Cat# 00202341A8BS Glycerol-3-phosphate dehydrogenase (GDH) Roche Cat# 10127752001 GST protein, tag-free Sigma Aldrich Cat# SRP5348 Human recombinant CSE, GST-tagged From Prof. Bibli Hu et al.66 Human recombinant CSE From Prof. Ruma Banarjee Wedmann et al.67 BODIPY 581/591 C11 Invitrogen Cat# D3861 SF7-AM Cayman Chemicals Cat# 14623 Critical commercial assays DC protein assay Biorad Cat# 5000112 NAD/NADH-Glo Promega Cat# G9081 NADP/NADPH-Glo Promega Cat# G9071 PureLink RNA Mini Kit Invitrogen Cat# 12183018A Glycerol-3-Phosphate Assay Kit Sigma Aldrich Cat# MAK207 L-Lactate Assay Kit Sigma Aldrich Cat# MAK443 Oxaloacetate Assay Kit Sigma Aldrich Cat# MAK070 Pyruvate Assay Kit Sigma Aldrich Cat# MAK071 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific Cat# 84868 Deposited data Total proteome, persulfidome; Thermal proteome profiling data PRIDE repository PXD051587; PXD051609 (Continued on next page) e3 Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNA Seq data BioProject database PRJNA1178314 Raw data for graphs This paper Data S1 Experimental models: Cell lines Mouse: MEF WT (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Mouse: MEF CSE-/- (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Human: HUVEC N/A Human: HEK293T N/A Experimental models: Organisms/strains C. elegans Wild-Type Bristol N2 Caenorhabditis Genetic Center N/A C. elegans: strain MRF03 (cth-1(ok3319)V) Caenorhabditis Genetic Center strain VC2569 C. elegans: strain MRF09 mpst-3(tm4387)V MITANI Lab, National Bio-Resource; Project of the MEXT, Japan strain FX04387 C. elegans: strain CF1038 (daf-16 (mu86)I) Caenorhabditis Genetic Center N/A C. elegans: strain SJ4103 (zcIs14 [Pmyo-3::mitoGFP]) From A.M. Vizuete (The Institute of Biomedicine of Seville) N/A C. elegans: strain RB1373 (F47G4.3(ok1558) I) Caenorhabditis Genetic Center N/A C. elegans: strain RB1482 (K11H3.1(ok1733) III) Caenorhabditis Genetic Center N/A Male Wistar Rat Institute for Biological Research ‘‘Sinisa Stankovic’’, Belgrade, Serbia N/A Oligonucleotides siRNA directed against human CSE Eurogentec custom synthesis Recombinant DNA psPAX2 Didier Trono Lab Addgene Plasimd #12260 pMD2.G Didier Trono Lab Addgene Plasimd #12259 Cdt-mKO Riken, Japan Geminin-GFP Riken, Japan Software and algorithms R Environment The R Project for Statistical Computing https://www.r-project.org/ R studio RStudio, Inc https://www.rstudio.com/ PEAKS ONLINE Bioinformatics Solutions Inc. http://www.bioinfor.com/ ImageJ Schneider et al.69 https://imagej.nih.gov/ij/download.html Fiji Schneider et al.69 https://imagej.net/software/fiji/ BioVenn Hulsen et al.70 https://www.biovenn.nl/ GraphPad Prism 10.2.0 GraphPad Software https://www.graphpad.com FragPipe Kong et al.71 https://fragpipe.nesvilab.org/ DAVID Bioinformatics Database v6.8 Huang et al.72 https://david.ncifcrf.gov/ EigenMS R script Karpievitch et al.73 https://rdrr.io/bioc/ProteoMM/src/ R/EigenMS.R Origin, Version 2022 OriginLab Corporation, Northampton, MA, USA N/A ChemDraw 20.0.0.38 PerkinElmer Informatics, Inc N/A Adobe Illustrator 28.4.1 Adobe https://www.adobe.com/products/ illustrator.html BioRender https://www.biorender.com/ Oasis Yang et al.74 https://sbi.postech.ac.kr/oasis/ CellProfiler Stirling et al.75 SeamDock Murail et al.29 https://bioserv.rpbs.univ-paris-diderot.fr/ services/SeamDock/ Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e4

    Peptide Fractionation:

    Article Title: Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Triethylammonium bicarbonate buffer Sigma Aldrich Cat# 18597 Hydroxylamine solution Sigma Aldrich Cat# 467804 Trypsin Promega Cat# V5117 TMT10plex Thermo Scientific Cat# 90110 DCP-Bio1 Merck Cat# NS1226 Copper (II)-TBTA complex Lumiprobe Cat# 21050 L-ascorbic acid Sigma-Aldrich Cat# A92902 Formic acid Suprapur Supelco Cat# 111670 TCEP Sigma-Aldrich Cat# 646547 AQC Synchem Cat# s041 AccQ-Tag Ultra Borate Buffer Waters Cat# 186009283 Cyanine5 alkyne Lumirpobe Cat# A30B0 Laemmli (4X) buffer BioRad Cat# 1610747 b-mercaptoethanol Sigma Aldrich Cat# M3148 Western Blotting Luminol Reagent Santa Cruz Biotechnology Cat# sc-2048 Ponceau S Sigma Aldrich Cat# P3504 Hydrogen Peroxide Solution Sigma Aldrich Cat# 216763 Erastin Sigma Aldrich Cat# E7781 1S,3R-RSL 3 Sigma Aldrich Cat# SML2234 Thiazolyl Blue Tetrazolium Bromide Sigma Aldrich Cat# M5655 Methyl viologen dichloride hydrate Sigma Aldrich Cat# 856177 Sodium (meta)arsenite Santa Cruz Biotechnology Cat# sc-250986 Nile Red Sigma Aldrich Cat# 72485 Oil Red O Sigma Aldrich Cat# O0625 Bovine Serum Albumin Sigma Aldrich Cat# A7906 L-(+)-Ergothioneine Thermo Scientific Cat# J67861.03 L-Hercynine Sigma Aldrich Cat# SMB00978 DMEM Gibco Cat# 21969-035 DMEM, high glucose, no glutamine, no methionine, no cystine Gibco Cat# 21969-035 DMEM/F-12 Gibco Cat# 21041025 DMEM, no phenol-red Gibco Cat# 31053028 Endothelial Cell Growth Medium 2 (ECGM2) PromoCell Cat# C-22011 Fetal bovine serum Pan Biotech Cat# P30-3031 L-glutamine Pan Biotech Cat# P64-80100 Penicillin-streptomycin Pan Biotech Cat# P66-07100 Polybrene Santa Cruz Biotechnology Cat# 28728-55-4 Lipofectamine RNAiMAX Invitrogen Cat# 13778075 DPBS Sigma Aldrich Cat# D8537 Hoechst 33342 Sigma Aldrich Cat# B2261 L-Cystine Sigma Aldrich Cat# 30200 Pierce High Capacity NeutrAvidin Agarose beads Thermofisher Cat# 20357 Pierce NeutrAvidin Agarose beads Thermofisher Cat# 29200 DTAB Thermo Scientific Cat# A10761.09 Sodium disulfide Dojindo Molecular Technologies Cat# SB-02 Dihydroxyacetone phosphate hemimagnesium salt hydrate Sigma Aldrich Cat# 51269 (Continued on next page) Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER b-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate Sigma Aldrich Cat# N8129 Bio-Spin P-6 Gel Columns Bio-Rad Cat# 7326227 Lead(II) acetate trihydrate Thermo Scientific Acros Cat# 423841000 Pyridoxal 5’-phosphate hydrate Thermo Scientific Cat# A12323 Granulated agar BD Difco Cat# BD 214530 Bacto Peptone Gibco Cat# 211677 LB Broth (Lennox) Carl Roth Cat# X964.1 NaCl Carl-Roth Cat# HN00.2 Potassium dihydrogen phosphate Carl-Roth Cat# P018.2 Di-sodium hydrogen phosphate dihydrate Carl-Roth Cat# 4984.1 Potassium phosphate dibasic Euromedex Cat# PB0447-B Agarose D5 Euromedex Cat# D5-C Ethanol Fisher BioReagents Cat# BP2818500 TRIzol Reagent Invitrogen Cat# 15596026 Phenol:chloroform:isoamyl alcohol mix (25:24:1) Sigma Aldrich Cat# 77617 DMSO Carl-Roth Cat# 4720.3 Methanol, Optima LC/MS Grade Fisher Scientific Cat# A456-212 Trichloromethane/Chloroform Carl-Roth Cat# 7331.1 Acetone ROTIPURAN R99.8% Carl-Roth Cat# 9372.2 Acetonitrile Biosolve Cat# 00012041001BS Acetic acid ThermoFisher Cat# A/0406/PB15 Isopropanol Fisher Chemicals P/7507/17 Ammonia solution 28-30% Sigma Aldrich Cat# 5.33003 NaOH Carl-Roth Cat# 1CCX.2 Hydrochloric acid 1N Carl-Roth Cat# K025.1 Trifluoroacetic acid Biosolve Cat# 00202341A8BS Glycerol-3-phosphate dehydrogenase (GDH) Roche Cat# 10127752001 GST protein, tag-free Sigma Aldrich Cat# SRP5348 Human recombinant CSE, GST-tagged From Prof. Bibli Hu et al.66 Human recombinant CSE From Prof. Ruma Banarjee Wedmann et al.67 BODIPY 581/591 C11 Invitrogen Cat# D3861 SF7-AM Cayman Chemicals Cat# 14623 Critical commercial assays DC protein assay Biorad Cat# 5000112 NAD/NADH-Glo Promega Cat# G9081 NADP/NADPH-Glo Promega Cat# G9071 PureLink RNA Mini Kit Invitrogen Cat# 12183018A Glycerol-3-Phosphate Assay Kit Sigma Aldrich Cat# MAK207 L-Lactate Assay Kit Sigma Aldrich Cat# MAK443 Oxaloacetate Assay Kit Sigma Aldrich Cat# MAK070 Pyruvate Assay Kit Sigma Aldrich Cat# MAK071 Pierce High pH Reversed-Phase Peptide Fractionation Kit Thermo Scientific Cat# 84868 Deposited data Total proteome, persulfidome; Thermal proteome profiling data PRIDE repository PXD051587; PXD051609 (Continued on next page) e3 Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER RNA Seq data BioProject database PRJNA1178314 Raw data for graphs This paper Data S1 Experimental models: Cell lines Mouse: MEF WT (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Mouse: MEF CSE-/- (Male) From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) Sbodio et al.68 Human: HUVEC N/A Human: HEK293T N/A Experimental models: Organisms/strains C. elegans Wild-Type Bristol N2 Caenorhabditis Genetic Center N/A C. elegans: strain MRF03 (cth-1(ok3319)V) Caenorhabditis Genetic Center strain VC2569 C. elegans: strain MRF09 mpst-3(tm4387)V MITANI Lab, National Bio-Resource; Project of the MEXT, Japan strain FX04387 C. elegans: strain CF1038 (daf-16 (mu86)I) Caenorhabditis Genetic Center N/A C. elegans: strain SJ4103 (zcIs14 [Pmyo-3::mitoGFP]) From A.M. Vizuete (The Institute of Biomedicine of Seville) N/A C. elegans: strain RB1373 (F47G4.3(ok1558) I) Caenorhabditis Genetic Center N/A C. elegans: strain RB1482 (K11H3.1(ok1733) III) Caenorhabditis Genetic Center N/A Male Wistar Rat Institute for Biological Research ‘‘Sinisa Stankovic’’, Belgrade, Serbia N/A Oligonucleotides siRNA directed against human CSE Eurogentec custom synthesis Recombinant DNA psPAX2 Didier Trono Lab Addgene Plasimd #12260 pMD2.G Didier Trono Lab Addgene Plasimd #12259 Cdt-mKO Riken, Japan Geminin-GFP Riken, Japan Software and algorithms R Environment The R Project for Statistical Computing https://www.r-project.org/ R studio RStudio, Inc https://www.rstudio.com/ PEAKS ONLINE Bioinformatics Solutions Inc. http://www.bioinfor.com/ ImageJ Schneider et al.69 https://imagej.nih.gov/ij/download.html Fiji Schneider et al.69 https://imagej.net/software/fiji/ BioVenn Hulsen et al.70 https://www.biovenn.nl/ GraphPad Prism 10.2.0 GraphPad Software https://www.graphpad.com FragPipe Kong et al.71 https://fragpipe.nesvilab.org/ DAVID Bioinformatics Database v6.8 Huang et al.72 https://david.ncifcrf.gov/ EigenMS R script Karpievitch et al.73 https://rdrr.io/bioc/ProteoMM/src/ R/EigenMS.R Origin, Version 2022 OriginLab Corporation, Northampton, MA, USA N/A ChemDraw 20.0.0.38 PerkinElmer Informatics, Inc N/A Adobe Illustrator 28.4.1 Adobe https://www.adobe.com/products/ illustrator.html BioRender https://www.biorender.com/ Oasis Yang et al.74 https://sbi.postech.ac.kr/oasis/ CellProfiler Stirling et al.75 SeamDock Murail et al.29 https://bioserv.rpbs.univ-paris-diderot.fr/ services/SeamDock/ Cell Metabolism 37, 1–15.e1–e14, February 4, 2025 e4



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